The DEG datasets were uploaded to the IPA application and mapped to the Ingenuity Pathways Knowledge Base (IPKB).
For this procedure, a list of the Entrez IDs for predicted targets that were known to be differentially expressed based on our previous study [ 9] was uploaded to the web application WebGestalt Gene Set Analysis Toolkit Version 2 [ 21].
The Affymetrix gene expression data were uploaded to the IPA application (www.ingenuity.com), and a core functional analysis was performed to identify canonical pathways that were most significantly associated with the differentially expressed genes (using a cutoff of >1.5-fold differentiation and P < 0.05).
At the same time, the differentially expressed genes sequences of each group were uploaded to the Mercator web application to assign MapMan bins, and then differentially expressed genes between the four compared groups of related pathways were screened and analysed using MapMan software [ 50].
The use of such applications for the analysis of, often sensitive, user's data requires the data to be uploaded to the web server of the application provider.
The monitors inform the cyclists about their personal exposures as they move through the city, and the data can be uploaded to an application that compiles them into a pollution map.
They tap into my social media history and send daily reminders of my past postings, from pictures uploaded to Instagram, the photo-sharing application, to messages on Facebook and Twitter.
All results were subsequently uploaded to a central database using the WiPam application [ 32]. Background characteristics included variables such as sex, age and socio-demographic status.
With the service, enterprtise applications are uploaded to Ravello where it then runs in the cloud for testing.
Proteins with fold change values of 1.5 above and -1.5 below were uploaded into the application and analysis was performed to generate networks and canonical pathway analysis.
The list of human homologs that correspond to the 50 protein-coding pig genes differentially-expressed was uploaded into the application.
