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When testing the latest mouse oligonucleotide probe collections of Illumina, Agilent and the RNG/MRC (Le Brigand et al., 2006), we found that, respectively, 33.4%, 50.3% and 79.2% of oligonucleotides were specific against Refseq 28 database, demonstrating the need to regularly update the specificity of probes.
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Thus, more accurate interpretation of microarray data requires parallel updating of the specificity of GeneChip probes.
Why update the saucepan?
Then update the formula.
Investigating these and other attributes in combination with each other may help to identify specific annotations that need to be updated, and to increase the specificity of CvManGO for finding genes that can be updated with novel functions.
The policy was recently updated to add specificity to the care of Alzheimer's patients, said Reingold.
All candidate probe sequences were tested against the NCBI nucleotide database (November 2005) by BLASTN to update their annotations and confirm the specificity of each probe.
Following this exercise, sensitivity of the expanded Approach 3 was improved: 0.581 (0.558 – 0.605) compared to 0.48 (0.456 – 0.504) for the original Approach 3. The specificity of the updated Approach 3 was reduced: 0.558 (0.527 0.58989) compared to 0.62 (0.59 – 0.65) for the original Approach 3. Changes to the algorithm focused on specificity also can be identified and implemented.
However, sequence variation among the different enterovirus groups can lower the specificity and PCR-based assays should regularly be updated using latest sequence information.
In order to increase the specificity and reduce the variability of ASD diagnosis, the updated DSM-V manual [ 6] proposes significant revisions of autism classification and diagnostic criteria.
You can taste the specificity.
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