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(See George Packer's new book, "The Unwinding," for an eloquent treatment of how we got there).
However, we still found no unwinding for CTP, GTP or UTP under these conditions.
This area of investigation is at its infancy; however, one known difference in the behavior among the known G4 DNA helicases is their polarity of unwinding (for review, see [ 4]).
These agents target the DNA in cancer cells and induce DNA damage either directly through DNA adduct formation (for example cisplatin) or indirectly via inhibition of DNA synthesis (for example gemcitabine and 5-fluoruracil) or DNA unwinding (for example etoposide).
Unwinding for the night, workers described the arduous work at the site, constricted by bulky protective suits and suffocating masks.
The slides are left in this solution for DNA unwinding for 25 min.
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After lysis, the slides were washed with distilled H2O, transferred to an electrophoresis unit containing freshly prepared electrophoresis buffer (500 mM EDTA, 200 mM NaOH, pH13.0), left for unwinding of DNA for 45 min, and the cells were electrophoresed for 15 min at 15 V.
Perhaps RecQ is not needed for the RecA/F/J- and SOS-dependent TLD pathway in UvrD+ cells (Fonville et al. 2010a) because UvrD can substitute for RecQ, for example, in unwinding DNA for RecJ single-strand exonuclease activity.
These molecular associations may target the SF2B helicase to a DNA structure requiring it activity and activate the SF2B helicase either for DNA unwinding or for remodeling of nucleoprotein complexes.
Brr2 is required for unwinding of U4/U6 during spliceosome activation [ 38, 39] and also for separation of U2/U6 interaction in disassembly of the spliceosome [ 40].
Because eIF4E is the limiting factor for eIF4F assembly, it determines the availability of eIF4F for unwinding of mRNA secondary structures [20], [21].
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