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So they raise their voices until the volume of their own voices equals that of callers'.
First off, don't hire PR help until the volume of inbound requests by press are simply too much to handle without help.
After adding all the syrup (16.0 L), the process was terminated until the volume of permeate reached 14.0 L (2.0 L syrup left in the tank).
Subsequently, the protein solution was diafiltered (3,000 MWCO) using 500 ml of 0.6 M NaCl solution at pH 6.0, until the volume of the solution was 20 ml.
The (10× dNTP Mix) mixture was prepared by using an equal dNTP concentrations as follows: 2 µL of dATP (0.2 mM), 2 µL of dGTP (0.2 mM), 10 µL dCTP (1 mM), 10 µL dTTP (1 mM) and the distilled water was added until the volume of 100 µL.
The pH of the mixture was adjusted to 6.8 with a 0.1 N HCl solution, stirred continuously for 30 min at 25°C and then centrifuged at 10,000 rpm for 10 min. The supernatant was filtered using a stirred cell with a 3,000 MWCO membrane until the volume of protein solution was 10 ml.
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The excess biotin was removed by spinning the filter at 12,000 G until the volume on top of the filter had been reduced to 0.25 the original volume.
The speed of data generation is astonishing from now until 2020, the volume of the digital data is expected to approximately double every two years, and 90% of the data available to us today were generated in just the last two years.
Tumors were measured regularly using vernier caliper until they reach the volume of 200 mm.
Using air, blow up white 12in balloons until they occupy half the volume of the space.
This process worked well enough until roughly 2004, when the volume of transactions exploded.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com