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Then 25 mL of HCl was added, and deionized water was added until the total volume of the solution was 250 mL.
The clear supernatants were collected and concentrated by additional centrifugation in 10K Centricon tubes (Millipore, Billerica, MA) at 4,000×g until the total volume of the supernatant fractions were reduced down to 1.5 ml.
When necessary, distilled water was added to until the total volume of liquid was 5.00 mL.
To the mixed solution of A and B, distilled water was added until the total volume was 100 ml.
Each dose was administered in volumes of 2-2.5 ml perfluorocarbon per inspiration, requiring approximately 4 min until the total volume of 7.5 ml/kg was applied.
The product solution was ejected from the outlet into a centrifuge tube containing 1 mL of acetonitrile until the total volume reached 1.3 mL.
Similar(51)
Nonetheless, it is interesting to note that the isotopic composition of siltstones in the sequence continued to show εNd values of around − 15, at least until around 6 Ma indicating that the total volume of material coming from the Inner Lesser Himalaya did not dominate over the Greater Himalayan material seen before that time.
The total volume ( plus the evacuated volume) is.
So, the total volume has gone up.
Adjust the total volume with 20% ethanol to twice the volume of the completely settled beads.
The injection volume was ~28 48 pl (1 3% of the total volume of the egg).
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