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In this work a Self Organized Radial Basis Function Network (SRBFN) is designed to focus on the myocardium in an iterative process until the total extraction of the myocardium from the noisy background is achieved.
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All the office myomectomy techniques until now were based on the total extraction of the fibroid's tissue from the uterine cavity.
Additional file 2: Figure S1c reveals that the total extraction yield was positively and significantly increased by the liquid solid ratio until the ratio was beyond 8 mL g−1; after that, the yield was almost unchanged.
The enzymatic extract (EE) was obtained by filtration of the cultures using filter paper (Whatman No. 4), and stored at −20 °C until it was analyzed, while the mycelium was rinsed with 0.9 % NaCl and stored at −70 °C until the total RNA extraction procedure was conducted or used for biomass (X) determination as difference of dry weight (g/L) (Additional file 1: Figure S1).
Half of the organs (n = 5) were preserved in 10% formalin diluted with phosphate buffered saline for histology analysis, while the other half (n = 5) were snap-frozen in liquid nitrogen and stored at -80°C until the total RNA extraction process for gene expression analysis.
The total RNA extraction from the pretreated stool sample was performed using commercial RNA extraction kit.
The current extraction rate of, coupled with the future extraction rate of for the project, is expected to produce a total extraction rate of.
The total RNA extraction was previously described [ 57].
The total RNA extraction was previously described in [ 68].
Many critics at the time responded as if this "new age baroque" style and use of melody were not mere aural toothache but total extraction.
Tissue samples were immediately frozen in liquid nitrogen and kept at −80°C until the extraction of total RNA.
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