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The label of "Not to open until the time of use" should also be included.
At the time of resection, samples were snap-frozen in the operating room in liquid nitrogen and then stored at minus 80°C until the time of use.
Keeping the surfaces of the membranes hydrophilic until the time of use both ensured that the biopolymer gel filled the holes in the membrane, and reduced formation of air bubbles in these holes.
All the extracts were lyophilized and kept in tightly closed glass containers and stored at -80°C until the time of use.
They were treated with a protease inhibitor cocktail (Roche, Indianopolis, IN), Mini, ethylenediaminetetraacetic acid (EDTA -free), cEDTA -free for 10 minutes at 1,000 × g and the supernatant was aspirated and stored at -80°centrifuged time oforse.
After incubation for 24 h at 37°C on a shaker at 200 rpm, the cultures were centrifuged at 8000 g for 10 min at 4°C and the supernatants obtained were stored at -20°C until the time of use.
Similar(53)
The management of the capital factor, from acquisition until the time of its use, will require a decision-making process focused on quantitatively and qualitatively capital resources observed both in their origin sources and in their destination uses.
The plasma was then decanted and stored at -20°C until the time of creatinine determination using manufacturer's instruction.
Women were asked at the baseline questionnaire whether they had ever used oral contraceptives, their duration of use up until the time of recruitment, and the age at first use.
To determine the pre-mainshock stress, all events from the beginning of the catalog (January 1997 for NIED, January 1977 for GCMT) until the time of the mainshock are used.
Twenty seven right-handed male volunteers, including 14 chronic heroin users [HU, heroin using (from the time of their initial heroin use until the time of scanning) for 7.11±2.82 years, range from 2 to 10 years] and 13 non-addicted controls (CN), participated in the present study.
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