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Hemizygous VAPΔ166 males survive until the third instar larval stage, but most of them die as pupae and very few "escaper" males are observed on eclosion [12].
Larvae were raised on fly food supplemented with all-trans retinal (Sigma, 100-200 nmol added daily on top of vials containing ∼5 ml of standard medium) until the third instar stage, then dissected in HL3 buffer [30].
These larvae survive primarily on maternal HP1a until the third instar stage after which they die without pupating.
WCR neonates were applied to corn roots and allowed to feed until the third instar, at which point carcass material was harvested from 10 larvae per replicate.
Cells in medullary and cortical zone divide actively until the third instar, when cells of the medullary zone become proliferatively quiescent (Jung et al., 2005; Mandal et al., 2007).
Larvae were then reared in insectary under standard conditions (25 ± 1°C, 80 ± 10% relative humidity and 12 h light/dark cycle) until the third instar stage for species identification.
Similar(51)
The larval development in O. rimosa is faster (6 days until emergence of the third instar larva from the host) and has higher survival rates (de Vries & Lakes-Harlan unpublished results).
The serosa wraps the developing embryo until the first instar larva stage and invades the host tissues by microvilli projections and form a placenta like structure able to divert host resources and allowing nutrition and respiration of embryo.
gambiae were reared in a dark-bottomed pan parallel to counterparts reared in a white-bottomed pan until the fourth instar.
Male and female larvae were dissected at the end of the third instar.
(3) The biotransformation rate of silkworm larvae between the third instar and the fifth instar could reach above 70%.
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