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(c) Mix until the sample is completely dispersed (check visually).
This process is iterated until the sample is exhausted.
Mix by brief vortexing or flicking the bottom of the tube until the sample is thoroughly resuspended.
Sampling will be by consecutive selection in accordance with the inclusion-exclusion criteria, for a period of 12 months until the sample is complete.
Use of formalin-fixed paraffin-embedded (FFPE) tissue in a predictive biomarker assay introduces additional challenges, one of which is ensuring the stability of the analyte after sectioning until the sample is analyzed.
An alternative to cryo-sectioning currently being explored is focused ion beam milling, in which material is removed from the surface of a frozen specimen by irradiation with a beam of gallium ions, until the sample is thin enough for TEM.
Similar(53)
The crosshead speed was set at a fixed rate of 2 mm/min, and the load was run until the sample was broken.
This process was repeated until the sample was clean.
Each sample was filtered through the MinElute column in aliquots until the sample was exhausted.
The excess was removed with several washes until the sample was slightly stained.
Supernatants were collected, and centrifugation was repeated several times, until the sample was clear of debris.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com