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After removing the whitening gel, the teeth were rinsed, dried, and kept in artificial saliva until the next procedure.
Porcine cartilage specimens were initially incubated under physiological conditions (37°C, 5% CO2) in physiological saline (Otsuka Pharmaceutical Factory, Tokushima, Japan) until the next procedure.
Two types of peanut plant organs, immature pod and leaf, were collected by immediate immersing in liquid nitrogen after removing it from the whole plant and stored at -80°C until the next procedure of total RNA isolation.
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The next patient; the next procedure.
Unless otherwise stated, brief centrifugation up to 1000 rpm in Juan MR 23i was applied after addition of a solution or before implementation of new steps, and all the PCR and enzymatic incubation programs were set to hold at 4°C indefinitely at the end until a next procedure.
Thus, for each patient, three times were recorded: the operating time, the total OR time (from start of preparations until room ready for next procedure) and the time at the recovery room until discharge.
Immediately after obtaining PEEPol, PEEP was increased again up to 20 cmH2O for one minute in order to restore lung volume and then it was brought back to PEEPol, which was maintained for the next two hours until the next scheduled optimization procedure.
The supernatants were discarded and the pellets frozen at -70°C until the next step in the procedure.
Thereafter, patients ambulated daily until the next excision and grafting procedure if clinically stable.
The channel reestimation and detection procedure continued until the next pilot sequence started.
Government officials say that Microsoft's request will require months of hearings and procedures that could stall the case until the next administration takes office.
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