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The culture was maintained without additional growth factor supplementation or culture media change over the course of a week from initial plating until the next passage for secondary spheroids.
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As does the next passage.
Where might the next passage be?
Infected cells were incubated until a cytopathic effect (CPE) was observed in 80%% of the monolayer, and collected supernatants were used for the next passage.
The supernatant from each passage became the inoculum for the next passage.
Then, supernatants were collected and used for the next passage of cells.
Trypsinized cells were added to the next passage at 1 × 106 cells/flask.
All cultures were grown at 37°C for 24 h before the next passage.
Read the next passage for teaching the off command, then continue with the steps below it.
These are contextualized in the next passages.
The same medium was used throughout the next passages.
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CEO of Professional Science Editing for Scientists @ prosciediting.com