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On day 11, 10 µl of 0.0002 ABS600 of exponentially growing cultures of appropriate bacteria were added to each well and incubated at 25°C until the indicated time points.
LNCaP cells were grown until the indicated time points, and then cells were harvested for analysis of PCA3 expression.
eGFP+ cells were then cultured in human ESC differentiation medium containing 1% KOSR and 100 ng/ml VEGF-A (Peprotech) until the indicated time points.
Mice were pretreated with single daily doses of GGA at 600 mg/kg for 7 days before BLM administration and then until the indicated time for collection of samples.
Similar(56)
After washing, the infected cultures were incubated at 37°C until the indicated times.
After irradiation, cells were recovered in CGM for the indicated time until harvested.
The indicated strains were tested for Ypk1 T662 phosphorylation at the indicated time points after PalmC treatment.
One hundred microliters of supernatant of culture medium was collected at the indicated time points and stored at −80°C until the sample concentration was determined.
Culture supernatants or total RNAs were harvested at the indicated time after treatment and stored at −80°C until assayed.
Cook al dente, until firm to the bite, following the cooking instructions on the package but checking the pasta a minute or two before the indicated time.
The same cells were observed at the indicated time point.
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CEO of Professional Science Editing for Scientists @ prosciediting.com