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Fresh faeces was collected from the mice and stored at -80°C until suspended in physiological saline and cultured on MRS agar plates containing 5% ethanol (Solveco).
When DMSO-dissolved curcuminoids were added to the aqueous protein solution, much of the curcuminoids precipitated and the precipitate was vortexed until suspended.
A 15 μl droplet of proteinase K (1% solution in 10 m M Tris (pH 8.5) and 1 m M EDTA) was added to the relevant areas on the section and the cells scraped with a fresh scalpel blade until suspended in the droplet.
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CEO of Professional Science Editing for Scientists @ prosciediting.com