Paul did not shoot until sinking a long 3-pointer with 4 minutes 20 seconds remaining in the first quarter against the Nets.
It was cryoprotected in 30% sucrose until sinking, immersed in OCT, flash frozen in dry ice cooled isopentane, and cryostat sectioned on to slides at 20 µm.
Brains were removed and kept in the cryoprotecting liquid overnight or until sinking.
Brains were removed and postfixed in the same fixative for 2 h, and then immersed into 30% sucrose in 0.1 M PB until sinking (20 40 h).
The excised brains were post-fixed in the same fixative for 2 h, then transferred to 20% and then 30% sucrose in 0.1 M PB (pH 7.4) sequentially at 4°C until sinking.
And the Dynamo are a pretty decent defensive team, or were until Vancouver sunk their teeth in.
Ramming their own vessel into the migrant boat over and over, they waited until it sunk completely before abandoning their human cargo to the caprices of the sea.
Then they were placed in 0.5 M sucrose-PBS (pH 7.4) at 4°C until they sunk, followed by embedding in OCT compound at −20°C.
After washing in 1x PBS, brains were transferred into 25% sucrose until they sunk.
