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The final solution was removed from the tube, filtered through a 0.7-μm Minisart® filter, and collected in a closed glass container that was kept at 60 °C (to prevent gelation) until subsequent use.
DNA pellets were air dried and stored at -80°C until subsequent use for labeling.
Extracted DNA was stored at −20°C until subsequent use in different PCR reactions.
The remaining cDNA was frozen at –80°C until subsequent use.
Extracted total RNA was stored at −80°C until subsequent use.
The RNA samples were stored frozen at -20°C until subsequent use in real-time RT-PCR procedures.
Similar(53)
All videos were compressed using the Radius Cinepak Codec and kept at full length and size until subsequent analysis.
After finishing the periodic discovery, D2D devices start dozing until subsequent discovery period starts.
The patient was followed until subsequent death or discharge.
Samples were stored at −80°C until subsequent analysis.
Urine specimens were frozen until subsequent analysis of electrolyte concentrations.
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