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The aspirate was frozen at −80°C until subsequent testing by a multiplex PCR/DNA hybridization assay that detects RSV genotype-A (RSV-A), RSV-B, and 22 other respiratory viruses (Infiniti RVP assay; Autogenomics, Carlsbad, CA, USA) (14 ).
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All operations were carried out under sterile hood, and the extracts were aliquoted for subsequent testing and stored at −20°C until use.
On testing days, similar size and sex mice were thawed and warmed by electrical heaters until skin temperature was 38 ± 1°C before injection and subsequent testing.
Nine-week-old male C57Bl/6J mice were group housed until they underwent stereotaxic surgery, after which they were singly housed throughout all subsequent testing, at a constant temperature (22 ± 1°C) and 12-h light/dark cycles.
Subsequent testing confirmed that she was infected, Mr. Smith said.
In subsequent testing over the weekend, the train-detection system "intermittently failed," the safety agency said.
But subsequent testing showed that air pollution levels were on the rise again inside the building.
Subsequent testing revealed the fact that Washington was infected with HIV.
The damage of a low score in 10th grade leads these students to spend most of their high school career preparing for subsequent tests until they have taken way too many without the results they intended.
If the number of repetitions until failure was different from 10, several trials of a maximal repetitive set until failure were performed on different days with lower or higher loads during subsequent test sessions, in order to determine the load leading to failure in exactly 10 repetitions.
Subsequent tests and CAT scans confirmed the worst.
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CEO of Professional Science Editing for Scientists @ prosciediting.com