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The samples were either analyzed immediately or stored at temperature below 4 °C until subjected to analysis.
After filtration through a 0.7-μm Minisart® filter, the resulting solutions were stored in closed containers at 60 °C (to prevent gelation) until subjected to electrophoretic analysis.
This solution was then filtered through a 1 μm glass microfiber filter (Whatman GF/C, Sigma-Aldrich, St . Louis MO, USA) and kept refrigerated under N2 atmosphere, until subjected to preparative high-performance size exclusion chromatography (HPSEC).
The slides were then fixed in acetone and stored at −80°C until subjected to immunohistochemistry using standard protocols.
with cover slips, and kept at 4°C in the dark until subjected to fluorescence microscopy examination.
Serum was incubated at 55°C for 1 hr to heat-inactivate complement and stored at −80°C until subjected to analysis.
Similar(42)
The material was stored at 4°C in plastic bags until it was subjected to DAH.
Samples were stored at −20 °C until use and subjected to two or fewer freeze-thaw cycles.
That process repeated until subjects thought the two lizards were moving at the same speed.
The tests lasted up to 30 minutes, or until subjects felt moderately sick.
The initial stage was performed unloaded, with power output progressively increased 10 watts/stage until subjects had achieved volitional exhaustion.
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