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The resuspended cells were kept on ice until sorting.
Cells to be sorted were resuspended in complete medium and kept cold until sorting.
4,6-Diamidino-2-phenylindole (DAPI) was added (0.2 μg/mL) to the single-cell suspension, and cells were incubated on ice until sorting (about 30 minutes).
Following incubation, cells were centrifuged, and the cell pellets were resuspended in 0.5 ml ALDEFLOUR assay buffer and maintained on ice until sorting.
Cells were resuspended in 1X PBS, pH 7.4, supplemented with 2% bovine serum albumin (Sigma-Aldrich) and kept at 4°C until sorting.
The cell pellet was resuspended in 200 μL PBS/1%BSA with 10 μL phycoerythrin- (PE-) conjugated goat anti-mouse IgM antibody (R&D Systems, Minneapolis, MN) for 30 min on ice, washed for 3 times, resolved in 400 μL of PBS, and kept on ice until sorting.
Similar(49)
Cells were washed and kept on ice until flow sorting into microtiter plates containing CellsDirect Lysis buffer.
I didn't get in until sort of 10 o'clock, but I sort of taped it and watched it.
I never had mates I could call or live with until I sorted myself out," he says.
This week some German politicians muttered that Iceland should not get in until it sorted out its economy.
We got them all a beer they stayed until we sorted them out with some money".
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