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It was thoroughly agitated on a hot plate with suitable stirrer until solubilized.
The mixture was vortexed and incubated at 65°C until solubilized.
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Fmoc-Cys(Trt -OH (Novabiochem) was dissolved aTrt -OHar equivaleNovabiochemith a small amount of DMF added until fully solubilized.
The solution was sonicated in the water bath for 5 minutes at room temperature until is fully solubilized.
The Cry proteins are inactive until they get solubilized by proteases in the insect's midgut at high pH (>9.5), releasing proteins called δ-endotoxins [ 2, 3].
The O.C.T. Compound was removed from the slide by incubating the slide in a Petri dish containing deionized water, until the media was solubilized, and the sections were detached from the microscope slide.
For RNA extraction, cancer cells were re-suspended in 400 µl RNA lysis buffer supplemented with 300 mg proteinase K (QIAGEN, Valencia, CA, USA) and incubated at 60°C for 16 hours until the tissue was completely solubilized.
The tubes were placed in a water bath at 80°C until all the tissues were solubilized.
Lysis was carried out at 60°C for 24 hours until the tissue was completely solubilized.
Finally, products were dissolved in dimethyl sulphoxide (DMSO) (Sigma Aldrich, USA) as a stock of 50 mg/ml and sonicated until the powder was fully solubilized.
Cells were then incubated in 1 % SDS solution until the dye was completely solubilized and absorbance was read at 570 nm.
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