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Both species were maintained in the presence of Symbiodinium until sampled 6 days (A. palmata) or 8 days (M. faveolata) later.
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Examples of these responses included "indefinitely" and "until samples are depleted".
After a second incubation at 65°C (until samples were thawn) the mixtures were centrifugated for 2 min at 12000×g.
Three mice were taken down at each time point, and biological samples were frozen at −70°C until sampling.
Thereafter, the mice were kept in preoperative conditions (24±2 °C) until sampling.
After pricking, larvae were individualized in 96 well plates containing flour and 5% yeast until sampling.
Group II was then maintained for three weeks in SW until sampling.
Antarctic octopods were caught using bottom and Agassiz trawls and kept in temperature controlled aquaria until sampling.
Subjects were instructed to avoid washing hands for at least one hour before the end of the flight until sampling.
Aliquot in 4 × 1.5 mL skirted microcentrifuge tubes was then stored in a –45 °C freezer until sampling.
They did to a bunch of elementary and high school students -- until they sampled the drinks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com