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Due to be replaced by James Taylor for the second half of the match, Wessels made the most of his limited appearance time and seemed well set for three figures himself until run out by a direct hit from Woakes at cover.
The venous blood serum samples for the cytokine analyses were stored at -80°C until run.
Stained samples were kept on ice in the dark until run on the flow cytometer.
The samples were frozen after the blood draw, and stored at −80°C until run.
The strips were then stored at −80 °C until run in the second dimension (SDS PAGE).
For paraquat treatment, paraquat (Sigma) was dissolved in water at a concentration of 15 μM and plants were sprayed once with this solution until run off.
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No one can even say for sure if the musical will be two and a half hours long, as expected, until run-throughs start.
The sprays were applied until run-off.
An aqueous solution of 1 mM JA was sprayed on the flowers until run-off and the same amount of tap water was sprayed on control plants.
Stems were directly treated with a solution of 10 mM CuCl2 until run-off point using a commercial garden sprayer.
Alethea solution was sprayed onto leaves until run-off using a pressurized airbrush, plants were air-dried and then returned to the glasshouse.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com