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Tumor samples were immediately snap frozen and stored at −80° C until retrieved.
One or two pieces from the periphery of the tumour masses – avoiding necrotic regions – were immediately frozen at –80°C until retrieved.
Paired-end reads were then end-repaired, A-tailed, and ligated to adapters, then amplified by PCR (98°C for 30s, 18 cycles of: 98°C 10s, 65°C 30 s, 72°C 30s, with a final step of 72°C 15 m and 4°C until retrieved).
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Instead of a tutorial level, a concept Amaya dislikes, the first level of the game gives the player two paths, one of which is blocked off until retrieving an item from the other path.
She pried open the window until he retrieved the keys.
I'd train my breath and learn to read sonar until I retrieved every lost blood vessel of you.
After I outgrew it, the blanket was stored in a drawer until I retrieved it near the end of my pregnancy and laid it next to all the things we'd bought in preparation for our son.
6.25pm GMT GOAL! Udinese 0-1 Liverpool (Henderson 22') Downing delivered the corner, Suarez tried to direct a header on goal but it was going wide until Suso retrieved it and cleverly laid it back to Henderson, who drilled it low and hard into the net from nine yards.
Some tugged at the strings and gave up, but two actually kept at it until they retrieved the sugar water.
Internet searches until May 31 , 2014 retrieved 152 potentially relevant papers; of them, 34 were excluded on the basis that did not report data on humans, 3 were in language other than English, 2 were excluded because they had a cross-sectional design, 3 because they reported data on secondary prevention and 82 were excluded on the basis of irrelevant research.
9 Dates of death until December 31 , 2009and causes of death until December 31 , 2008were retrieved from the Swedish Cause of Death Register.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com