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In this process, model parameters varied until recorded flow patterns were accurately simulated.
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Until recording technology arrived, hard evidence was limited to images of open mouths on walls and pots, and medieval singing manuals.
After labeling, the cells were washed with ND96 and kept on ice until recording.
Slices were stored for 45 min at 35°C and kept at room temperature until recording.
300-μm thick transverse slices were cut on a Leica vibratome (Leica VT1200) and the slices were left at room temperature in the holding chamber, until recording.
Horizontal hippocampal slices (400 µm) prepared from P21 to P32 animals were incubated in oxygenated ACSF at 31°C for 30 min, then stored at room temperature until recording.
Tetrodes were independently lowered until they recorded visually-driven extracellular action potentials.
Trainees who failed to achieve knowledge-based mastery in the first attempt sat for re-exam until they recorded mastery.
However, no written computations were recorded until much later.
The load, mid-span, deflection, and strains data were recorded until failure of the specimens.
The drop of initial CO2 pressure with time was recorded until a constant pressure was achieved at the equilibrium.
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