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Striatum was dissected on ice and rapidly frozen on dry ice prior to storage at −80°C until ready for monoamine analyses.
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The samples were stored at 4 °C until ready for flow cytometric analysis.
The prepared AuNP solutions were stored at 4°C until ready for use.
Samples for Western blot analyses were stored at −80°C until ready for analysis.
HeLa-TRPV4 cells were grown on 10-cm tissue culture plates until ready for harvest.
cRNA was stored at −20°C until ready for use.
Extracts were stored at -20°C until ready for analysis.
Cultures were stored at 4°C until ready for DNA preparation.
Samples were stored at -80°C until ready for use.
All samples were stored at – 70°C until ready for use.
DNAs were denatured at 95° for 10 min and placed at 42° until ready for hybridization.
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