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Seeds of A. artemisiifolia collected from wild populations in Woodstock, Illinois, were vernalized by sowing seeds in six growth containers containing compost (Pro-Mix, Red Hill, PA) and storing in a refrigerator at 4°C until ready for germination.
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The building seems to encourage growth, with a floral carpet growing ivy and a dirt table apparently ready for germination.
Judging from the cell arrangement and their ability to form protocorms upon germination, histodifferentiation has occurred and the 'protocorm body plan' is laid down ready for germination.
The prepared AuNP solutions were stored at 4°C until ready for use.
The samples were stored at 4 °C until ready for flow cytometric analysis.
Samples for Western blot analyses were stored at −80°C until ready for analysis.
HeLa-TRPV4 cells were grown on 10-cm tissue culture plates until ready for harvest.
cRNA was stored at −20°C until ready for use.
Extracts were stored at -20°C until ready for analysis.
Cultures were stored at 4°C until ready for DNA preparation.
All samples were stored at – 70°C until ready for use.
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CEO of Professional Science Editing for Scientists @ prosciediting.com