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Cells were split every five days until proliferation of control cells stopped.
The adult abdominal epidermis is derived from imaginal histoblast cells that remain quiescent until proliferation is triggered at pupariation (Madhavan and Madhavan, 1980).
HMEC lines were expanded in MBD170 serum-free basal medium (Life Technologies, Carlsbad CA, USA) until proliferation slowed and the cells entered the selection phase (passage 4 to 7).
Early passage HDF used in this study were at passage 15 and MTF at passage 3. HDF were cultured until proliferation ceased (monitored by daily microscopic observation) and incubated for 2 more weeks prior to harvest.
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For centuries on end, at least until the proliferation of gunpowder in the 15th-century West, catapults saw action as the early weapons of mass destruction.
Single and on a limited income, I never felt the need to drive a large car until the proliferation of S.U.V.'s.
Following this notion, the speckle localization of B-Myb might serve to transiently store B-Myb until cell proliferation resumes.
That would be expensive, and the American Congress is already balking at the cost of paying for fuel oil for North Korea (until two proliferation-compliant nuclear reactors are built in North Korea by an international consortium).
Facing difficulties already in persuading Congress to pay for 500,000 tonnes of heavy fuel oil a year (until two proliferation-compliant nuclear-power reactors are built in North Korea by an international consortium), America may be unwilling to pick up the bill.
You've registered the point once the Servant, early in the show, describes an archetypal woman "so beautiful that her image was replicated in infinite proliferation, until finally she could no longer be said to belong to herself alone".
For OSTs, the substrate with 70 min collecting time shows progressive rate of growth and proliferation until 14 days compared to other substrates and control as shown in Fig. 3b.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com