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For insulin, additional blood was collected in EDTA-containing tubes and centrifuged at 1,500g for 15 min; supernatant (plasma) was collected and stored at −80 °C until processing with an ALPCO Rat Insulin ELISA kit.
Tissue samples were harvested from each tree, and flash frozen and stored at −80°C until processing with the exception that combined bark and xylem tissue was cut into 1 cm sections prior to freezing.
Urine was collected into sterilized 100 mL polypropylene cups containing 125 mg ascorbic acid, kept cold with ice packs (0 4°C) until processing within 6 hr of collection, and then stored at –70 to –80°C.
Samples of exfoliated cervical cells were obtained from the cervical os with a cervical brush, which was stored in Thinprep® at -80°C until processing for HPV genotyping.
Cultures with CPE were stored at -70°C until processing for indirect sandwich ELISA.
Retrieved plates were stored in a cool box with icepacks until processing later the same day in the laboratory.
Processing began with a simple idea.
FTA cards were stored at room temperature with desiccant until processing.
It was shown that an excess of propoxy groups slows down the reaction kinetics but until 120 °C, the gelling time is longer than 8 minutes which is long enough for processing with conventional techniques.
The primary science event is not affected, since once event processing starts with the initial trigger pulse, a gate rejects further pulses until processing is complete.
Specimens were obtained with a cervical brush and stored in frozen conservative media until processing.
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CEO of Professional Science Editing for Scientists @ prosciediting.com