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The frozen samples were transported to the lab and stored there at −18 °C until preparation.
Within 2 min of euthanasia, m. soleus (120 180 mg) were harvested and put in pre-chilled tubes with isolation solution (100 mM sucrose, 100 mM KCl, 50 mM Tris-HCl, 1 mM K2HPO4, 0.1 mM EGTA, and 0.2 % bovine serum albumin (BSA), pH 7.4) and kept on ice until preparation.
Spleens were collected and stored in PBS until preparation.
Subsequently, pellets were flash frozen in liquid nitrogen and stored at −80°C until preparation.
Following manufacturer's recommendations, the filters were immediately infiltrated with RNAlater solution and stored frozen at −20°C until preparation of RNA.
Specimens were kept cool until preparation of the slides.
Similar(27)
All he can do, at least until preparations are in place, is watch from a command post in nearby Jacksonville.
Ironically, those changes meant that the British offensive would be resumed at a much later date than Auchinleck had proposed, as the impatient Churchill had to give way to Montgomery's determination to wait until preparations and training were completed.
The government lawyers argued that waiting for a targeted killing of a suspect "until preparations for an attack are concluded, would not allow the United States sufficient time to defend itself".
Samples were snap frozen in liquid nitrogen and kept at −80 °C until sample preparation for UHPLC-UV analysis.
In order to attenuate the invisible agent, he desiccated the spinal cords of infected animals until the preparation became almost nonvirulent.
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