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Of course, data alone has little value until it's analyzed.
The enzymatic extract (EE) was obtained by filtration of the cultures using filter paper (Whatman No. 4), and stored at −20 °C until it was analyzed, while the mycelium was rinsed with 0.9 % NaCl and stored at −70 °C until the total RNA extraction procedure was conducted or used for biomass (X) determination as difference of dry weight (g/L) (Additional file 1: Figure S1).
For this, supernatant from each well was collected every 24 h and frozen at −20°C until it was analyzed by RT assay for viral release as described previously [40].
Serum was stored at −80°C until it was analyzed.
Plasma was stored at −20°C until it was analyzed.
Then, the saliva samples were centrifuged, and the supernatant was stored at −80°C until it was analyzed.
An aliquot of 5 mls was removed from the first sample of pasteurized expressed breastmilk from each donor and was kept frozen at -20°C until it was analyzed.
Extracted DNA was eluted with 100 μl AE buffer (10 mM Tris, pH 8.5) and stored at -20°C until it was analyzed.
The cleared media was transferred to a sterile 1.5 microfuge tube and stored at -80°C until it was analyzed by ELISA for cytokine levels.
After centrifugation, the serum obtained was stored at −70°C in glass vials (prewashed with pesticide hexane grade) covered with a Teflon cap, until it was analyzed.
All the samples were kept at 4 °C until these were analyzed.
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