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The enzymatic extract (EE) was obtained by filtration of the cultures using filter paper (Whatman No. 4), and stored at −20 °C until it was analyzed, while the mycelium was rinsed with 0.9 % NaCl and stored at −70 °C until the total RNA extraction procedure was conducted or used for biomass (X) determination as difference of dry weight (g/L) (Additional file 1: Figure S1).
For this, supernatant from each well was collected every 24 h and frozen at −20°C until it was analyzed by RT assay for viral release as described previously [40].
Serum was stored at −80°C until it was analyzed.
Plasma was stored at −20°C until it was analyzed.
Then, the saliva samples were centrifuged, and the supernatant was stored at −80°C until it was analyzed.
Extracted DNA was eluted with 100 μl AE buffer (10 mM Tris, pH 8.5) and stored at -20°C until it was analyzed.
Similar(51)
Of course, data alone has little value until it's analyzed.
"Any (hot dog) they manufacture does not go out the door until it is analyzed as containing no Listeria monocytogenes, " said Adams.
Information was collected from the late 1970's until late 1981; over the next three years it was analyzed and extrapolations were made to the general population.
It was not until later data was analyzed that the NHC revised it to Category 1 intensity in the Saffir-Simpson Hurricane Scale.
"I waited until a biopsy was analyzed showing it was 100percentt cancer," he said.
More suggestions(15)
until it was done
until it was eclipsed
until it was discontinued
until it were analyzed
until it was incorporated
until it was finished
until it was rented
until it was interrupted
until it was dropped
until it was reassembled
until it was gone
until it was broken
until it was published
until it was excavated
until it was discovered
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