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Until further validation in human studies, the implications of this work for patient care remain unclear.
Therefore, these associations have to be interpreted with caution until further validation.
This suggests that the miRNA in question should not be annotated as orthologues until further validation studies have been completed.
After their discovery from the original training dataset and passing visual screening of heat maps, classifiers were still considered as candidates until further validation on an independently generated microarray dataset.
Until further validation research has been carried out in the context of educational practice, we caution against uncritical adoption of this measure, although we are optimistic it will prove useful.
Despite these promising data, however, the recent American Society of Oncology guidelines have stated that even the use of the US Food and Drug Administration-cleared CellSeassayassay in patients with metastatic breast cancer cannot be recommended until further validation confirms the clinical value of this test [ 72].
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All blood-based biomarkers reported until now need further validation.
Until more robust algorithms and further validation studies in critically ill patients become available, we should be aware that in conditions of haemodynamic instability, uncalibrated ABP CO systems may diverge from independent bolus measurements, particularly if a peripheral artery is used as this may underestimate or overestimate central blood pressure depending on the vascular tone.
These pioneering approaches were developed from personal experience and from findings in small patient groups but have been used until today, although often modified, without further validation.
Further validation analyses for the new instrument cannot be undertaken until data has been acquired from a range of facilities, a task out with the remit of the present study.
After reviewing the literature we found evidence that link most of the top-scoring miRNA pairs (until 10th rank) to aging and cardiovascular diseases and consider them reliable for further validation.
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