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Live species were harvested by centrifugation at 1700 g for 10 min and stored at −80°C until further treatment.
Transfected cells were rinsed 24 h post transfection with PBS and maintained in culture medium until further treatment.
Soon after sampling, gastrotrichs were extracted from the sandy substrata using a 7% MgCl2 solution [17], fixed in 95% Ethanol and stored at −20°C until further treatment.
Brains were dissected and stored in fresh EM fixative at 4°C until further treatment.
The sample was sieved through 120 μm mesh and stored in sterile distilled water at 2°C until further treatment.
Dead individuals were collected daily, stored in individually numbered plastic 1.5-mL vials and stored at −20°C until further treatment.
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Soon it was announced that he would be out until March 2014, following further treatment on his knee.
Treatment was administered every 2 weeks until disease progression or unacceptable toxicity, or until the patient declined further treatment.
Multi-surface or very extensive lesions in primary molars are best treated with preformed metal crowns because recurrent caries of teeth restored with these crowns do not occur and these restorations will last, without requiring further treatment, until the primary molars spontaneously exfoliate.
After completing the scheduled treatment, patients were followed up with no further treatment until confirmation of metastasis or recurrence.
Patients returned at 2-week intervals for further treatment until both sides of the face reached optimal correction.
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