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After one day of fixation at 4°C, the samples were washed with 5% glucose in 0.1 M cacodylate buffer which was exchanged every 10 days and stored at 4°C until further steps.
This process continued until further steps of the recruitment process (exclusion, refusal or enrolment) were completed.
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Isoelectric focusing was performed after passive rehydration at room temperature and run at 50 V 9.5 hrs, 250 V (rapid) for 15 min, 500 V (rapid), 1000 V (rapid) for 15 min, 10000 V (rapid) - 35000 V/hrs, and finished at 500 V until further step.
But he said India, which had already allowed commercial flights from Pakistan and moved its navy ships from Pakistan's coast, would do nothing until Pakistan took further steps to disrupt militants.
The group will not take any further steps until after its leaders return from Rome and its members gather in regional meetings and at a wider assembly in August.
Britain's foreign secretary, Robin Cook, said he delivered the message in talks with a delegation of three Zimbabwean ministers and made clear that Britain would "refuse to take any further steps until the next step is taken, and that is for the occupations to come to an end".
Analysts said the central bank's statement suggested that it was unlikely to take further steps until the government itself acted.
Whereas the Palestinians have turned to the international community for recognition and restitution, they will be wise to take no further steps until after the Israeli elections and a new government is formed.
There will be no further steps taken until the election outcome is known and the position on future funding is clear.
"As we have previously said, there will be no further steps taken until the election outcome is known and the position on future funding is clear," said a spokeswoman for ITV.
The cell suspension was immediately layered onto pre-chilled frosted glass slides (Fisher) pre-coated with 0.6% agarose and maintained in the dark at 4 °C until set, and for all further steps.
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CEO of Professional Science Editing for Scientists @ prosciediting.com