Sentence examples for until further sequencing from inspiring English sources

The basic scheme of an EST sequencing project relies on a cDNA library constructed from a tissue of interest under a particular condition from which randomly isolated clones are sequenced until further sequencing no longer yields an acceptable frequency of identifying novel cDNAs.

The LRRCE regular expression pattern was refined in iterative cycles until no further sequences were obtained.

This process was repeated with each newly identified set of plant LIM genes until no further sequences with significant similarity were identified.

In ProDom, the shortest sequence in a database is assumed to consist of a single domain; members of that domain family are built up using successive PSI-BLAST searches, and the process iterates until no further sequences can be clustered (Servant et al., 2002).

At the time of clinical evaluation, haplotype analysis was compatible with OTOF mutation; however, OTOF was negative for mutations and no further sequencing was completed until inclusion in this study.

When no further sequences could be added to the bin, a new bin was started with a remaining pairwise alignment, until no further sequences remained with sufficient quality alignments.

(iii) Further sequences of at least fs-full-len bases length are kept independent of their match score until the set of selected sequences contains at least fs-req-full such sequences.

(iv) Further sequences are kept if they cover the start and end of the gene as determined by the alignment positions gene-start and gene-end until at least fs-cover-gene such sequences have been found.

Thus, our results should be interpreted with caution until further verification of sequencing approaches plus larger and larger meta-analysis.

A series of searches, with different combinations of ingroup and outgroup taxa, were tested until further addition of taxa and sequences had no effect on topology.

Total genomic DNA was extracted from foot muscle tissue using the Dneasy™ Tissue Kit (Qiagen, Germany) following the manufacturer's protocol and stored at −20°C until further analyses.> Initially eight mitogenomes were sequenced, corresponding to two individuals per mitochondrial lineage, as inferred from 313 bp of cox1 by Becquet et al. [ 21] (and hereon referred to as " cox1 haplotypes").