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The received white powder was stored at −20°C until further experiments.
All drug solutions were stored at −20 °C until further experiments.
It is important to note that the locations displayed here may be caused by singularity issues in the model, and that they should be taken as assumptions of failure points until further experiments validate or invalidate them.
The crude extract was maintained at −20°C until further experiments were done.
The supernatant (cytosolic extract) was collected and stored at −80°C until further experiments.
Then the hearts were snap-frozen in liquid nitrogen and stored at −80 °C until further experiments.
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All aptamers were reconstituted in Tris-HCL (10 mM, pH 8.4) as 100 μM stock solutions and stored at -20°C until use for further experiments.
After 4 days of heating, the particle suspensions were refrigerated at 4°C until their use in further experiments.
This protocol was repeated up to three times until we got enough chondrocytes for further experiments.
Chemostat cultures are kept under completely controlled conditions and have a constant inflow of defined nutrients and an outflow of waste products that is monitored until steady state is reached before further experiments (here reduction of oxygen) are undertaken.
The reprogrammed cells remained stable for at least a month, and further experiments revealed that the capacity for reprogramming persisted until three days of age, but had been eliminated by three weeks of age.
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CEO of Professional Science Editing for Scientists @ prosciediting.com