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In brief, after collection, the blood samples were immediately centrifuged for 15 min at 3000 ×g and plasma immediately withdrawn and stored at −20°C until further assays.
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Supernatants were then removed and stored at −80°C until further assay.
Total RNA from tissue samples or from cells was stored at –80°C until further assay.
Cell suspensions were overlaid onto the transfection complexes and incubated at 37 °C for 48 h until further assay.
An aliquot was used to determine soluble protein concentration and the rest of the cytosolic extract was frozen at -20°C until further assay.
Immediately after irradiation, the irradiated cells and nonirradiated cells growing on different coverslips were placed face-to-face with a 3-mm gap and cocultured in a 35-mm dish with fresh medium until further assay.
DNA quality and quantity were examined with a Synergy H4 Hybrid Multi-Mode Microplate Reader (BioTek Instruments, Winooski, VT, USA) and then stored in aliquots at −20 °C until further assay.
All specimens and serum samples were stored at −80°C until used for further assays.
The samples were kept frozen at −70°C within one hour until they were further assayed.
The remaining eluted DNA from COBAS 4800 will be stored at −80°C until required for further assay.
Plasma samples were collected after centrifugation at 10000 rpm and stored at -80°C until they were further assayed.
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