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Samples were immediately placed on ice and centrifuged at 2000 g for 10 minutes and then plasma was frozen and stored at −70°C until further analyzes which were performed within three months after collection.
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Aerial parts were cut, air-dried and powdered in a grinding machine and stored in an airtight container until further analyzed.
Samples were stored at −20°C until further analyzed.
The plasma blood samples were centrifuged under refrigeration at 7000 rpm for 10 min and stored at −20°C until further analyzed by HPLC.
On the fifteenth days, the mice were bled, the blood was collected and centrifuged at 3,000 rpm for 10 min at 4°C, then stored at −80°C until further analyzed.
DNA was eluted with 50 μL Buffer AE, centrifuged at 14,000 rpm for 1 min, and then the DNA extract was stored at −20°C until being further analyzed.
Skeletal muscle specimens (tibialis anterior or quadriceps) from PED/PEA-15 transgenic or control mice were processed according to Paciello et al. Briefly, dissected muscles were frozen in isopentane pre-cooled in liquid nitrogen and stored at 80 °C until being further analyzed.
Now, the agency says, it needs until July 2011 to further analyze scientific and health studies of the smog rules and until April 2012 on the boiler regulation.
The samples were immediately stored at –80°C until they were further analyzed.
were further analyzed.
This report includes preliminary findings that are being expanded and further analyzed for future publication.
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