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Aerial parts were cut, air-dried and powdered in a grinding machine and stored in an airtight container until further analyzed.
Samples were stored at −20°C until further analyzed.
The plasma blood samples were centrifuged under refrigeration at 7000 rpm for 10 min and stored at −20°C until further analyzed by HPLC.
On the fifteenth days, the mice were bled, the blood was collected and centrifuged at 3,000 rpm for 10 min at 4°C, then stored at −80°C until further analyzed.
Similar(56)
Samples were immediately placed on ice and centrifuged at 2000 g for 10 minutes and then plasma was frozen and stored at −70°C until further analyzes which were performed within three months after collection.
DNA was eluted with 50 μL Buffer AE, centrifuged at 14,000 rpm for 1 min, and then the DNA extract was stored at −20°C until being further analyzed.
Skeletal muscle specimens (tibialis anterior or quadriceps) from PED/PEA-15 transgenic or control mice were processed according to Paciello et al. Briefly, dissected muscles were frozen in isopentane pre-cooled in liquid nitrogen and stored at 80 °C until being further analyzed.
The samples were immediately stored at –80°C until they were further analyzed.
Thus, they were not further analyzed.
were further analyzed.
Therefore, data involving Test 3 were not further analyzed.
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