The dried leaves were then ground into powder and kept at 25 °C until further analyses.
Such an interpretation, however, remains pure speculation until further analyses are completed.
The remaining sampled tissues were frozen in liquid nitrogen and stored at −80 °C until further analyses.
Plants were harvested on the 7 and 14 d of salt treatment and then stored at -80°C until further analyses.
The mice were kept with water containing antibiotics (1.1 g/L of neomycin sulphate) until further analyses.
The pancreatic juice samples were collected in tubes containing protease inhibitors and were immediately frozen until further analyses.
After extraction, DNA was quantified by spectrophotometery, checked for purity and stored at −35°C until further analyses.
Tissue was snap frozen in liquid nitrogen within 5 minutes after removal, and stored at −80°C until further analyses.
At the end of the clamp, mice were anesthetized, cardiac punctures were performed and hindlimb muscles were excised, freeze-clamped, and stored at −80°C until further analyses.
Upon completion of the clamp, the rats were killed with an overdose of sodium pentobarbital and TA muscles were quickly dissected and frozen in liquid nitrogen-cooled isopentane and stored at −80°C until further analyses.
The fibroblasts were stimulated with human recombinant CXCL13, 200 ng/ml (R&D Systems, Minneapolis, MN), with or without the ERK1/2 inhibitor (10 µM final concentration UO126 (MEK inhibitor, Promega, WI)), for 3 and 20 hours before storing cell pellet (mRNA analyses) and cell-free supernatant (MMP activity) at −80°C until further analyses.
