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Some voucher-supporters wish he had waited until experiments in places like Milwaukee had produced more evidence of the idea's benefits.
The belief that water was a fundamental substance persisted for more than 2,000 years until experiments in the second half of the 18th century showed that water is a compound made up of the elements hydrogen and oxygen.
The specimens were fresh-frozen and stored at a temperature of − 20 °C until experiments.
The work holds great promise, he says, but it may move slowly until experiments with brain-dead patients gain greater acceptance among clinicians.
In April all subjects reported to the laboratory at sea level in Copenhagen three days prior to the experiments and were not allowed to leave the building until experiments had finished.
If the heart was used for biochemical experiments, it was dissected into different regions of ∼5×5 mm chunks, snap frozen in liquid nitrogen and stored at −80°C until experiments.
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Both sizes of particles were preserved in desiccator until experiment.
All blood samples were collected in EDTA tubes, immediately centrifuged at 2000g for 10 min to obtain plasma, frozen and stored at −80 °C until experiment.
100 µl of media was exchanged each day and until experiment completion (72 hrs).
Freshly enucleated pig eyes were obtained from a local slaughterhouse (Etablissement Guy Harang, France) and stored in Hanks' Balanced Salt Solution (Sigma) until experiment.
Almost all individuals remaining inside intact domiciles (IH1 and IL1) survived until experiment 1 was terminated at 182h, regardless of humidity.
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CEO of Professional Science Editing for Scientists @ prosciediting.com