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Both sizes of particles were preserved in desiccator until experiment.
All blood samples were collected in EDTA tubes, immediately centrifuged at 2000g for 10 min to obtain plasma, frozen and stored at −80 °C until experiment.
Environmental waters used to prepare the photo degradation experimental solutions were filtered twice using a 0.2-micron 47 mm glass fiber filter to remove any particles and microorganisms and then stored in the dark at <4°C until experiment was performed, typically within a week.
100 µl of media was exchanged each day and until experiment completion (72 hrs).
Freshly enucleated pig eyes were obtained from a local slaughterhouse (Etablissement Guy Harang, France) and stored in Hanks' Balanced Salt Solution (Sigma) until experiment.
Almost all individuals remaining inside intact domiciles (IH1 and IL1) survived until experiment 1 was terminated at 182h, regardless of humidity.
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Some voucher-supporters wish he had waited until experiments in places like Milwaukee had produced more evidence of the idea's benefits.
The specimens were fresh-frozen and stored at a temperature of − 20 °C until experiments.
The work holds great promise, he says, but it may move slowly until experiments with brain-dead patients gain greater acceptance among clinicians.
The methanolic extract of the plant (1.53 g) was obtained and stored at 4°C until experiments.
Venom was diluted in physiological saline (1 mg/mL) and stored at −20°C until experiments.
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