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The doctor said he could not be certain until examining the tissue that would be removed during surgery, but because of its size he believed the tumor was malignant.
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She added a cautionary note: "Until examined by scholars, however, the true literary importance of this particular collection is yet to be determined".
All tissue samples were placed in sealable, sterile containers and kept refrigerated at 4°C until examined (usually within hours or days).
One colony for each observed morphology was picked up, stored in Cystine Triptose Agar (Difco) and sent to the reference microbiology laboratory in Italy, where they were checked for purity and stored at −70°C in glycerol 20% until examined.
All samples were centrifuged, and stored as serum at -20 C, until examined.
Composite samples were immediately fixed with Lugol's iodine, then stored at 4°C until examined.
One part was frozen in liquid nitrogen, and stored at -80°C until examined.
Feces were stored at 4°C until examined within 6 12 days by quantitative sugar flotation and light microscopy (8 ).
Serum was extracted from whole blood by centrifugation at 3000 rpm for 15 min at 4 °C and stored at −20 °C until examined.
They were then washed 3 times in PBS, mounted on to slides with Vectashield mounting medium (Vector Laboratories Inc). and stored in the dark at 4 °C until examined.
All specimens were stored in liquid nitrogen or in 70-95% ethanol, at room temperature or in a freezer at −20°C until examined.
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