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These processes were repeated until elimination of all primers having stretches of 8 bp or more matching with other primers in the same cocktail.
The influence of Qfhs.ndsu-3AS in the T. dicoccoides accession 'FA-15-3′ (Syn. 'Israel A') was not revealed until elimination of the suppression activity of the 2A QTL by using the chromosome substitution lines (Stack et al. 2002).
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The resulting slurry was homogenized periodically and kept in a hood until complete elimination of solvent.
Were animals transcardially perfused until complete elimination of blood cells before fixation?
The medium was changed every 3 days until complete elimination of cells in the control well (non-transfected cells).
The extracts were concentrated under vacuum (Buchi RE 215) until complete elimination of the organic solvent and subsequently freeze-dried for water elimination, providing dichloromethane (DHE), ethanolic (EHE) and aqueous hot extract (AHE).
The extracts were concentrated under vacuum (Buchi RE 215) until complete elimination of the organic solvent and subsequently freeze-dried for water elimination, providing dichloromethane (DE), ethanolic (EE) and aqueous (AE) extracts.
Variables with the lowest predictive value were deleted from the model until further elimination of a variable resulted in a statistically significant lower model fit estimated with the likelihood ratio test (p ≤ 0.20).
The ethanolic extracts resulting from three consecutive extractions were mixed together then concentrated under reduced pressure in rotary evaporator until complete elimination of solvent, and stored in a freezer at −20°C.
While blood concentrations of an antimalarial drug (or drugs) exceed the MIC in malaria, parasite numbers decline, so a generally efficacious regimen is one that maintains blood concentrations above the MIC in all patients until complete elimination of even the most drug-resistant naturally occurring infection.
Mothers and infants with contraindications for breastfeeding will be excluded: a. Mothers' contraindications: HIV positive, substance abuse, chemotherapy, radioactive isotope treatments until the elimination of the isotope from the mother's body, active tuberculosis, active chickenpox, active Herpes lesions, Chagas disease.
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