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Unsporulated oocysts (2 × 10) and sporulated oocysts (5 × 10) were resuspended in 1 ml TRIzol® Reagent and one volume of glass beads were added to the sample, which were then vortexed for 1 min intervals until disruption of oocyst was confirmed by bright field microscopy.
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Disasterproof the Home Most people who have lived for years in rural and semirural areas where power lines are above ground are quite used to interruptions in electrical and telephone service, and they are usually equipped to cope reasonably well until disruptions are repaired.
The Agrarians were the backbone of government coalitions until the disruption of the republic during World War II; from its ranks came Antonín Švehla (prime minister, 1921 29) and his successors.
For harvesting, after 15 min of centrifugation at 4700×g and 4 °C, the cells were quick-frozen with liquid nitrogen and stored at −20 °C until cell disruption and purification or immobilization.
Until this disruption, he made enough money to support a wife and two daughters, one of whom is getting ready for college.
Campaigning was due to start on 13 April for the second round, until its disruption as a result of the coup d'état.
These plays start in 1727, when Fielding first worked on Love in Several Masques, until the disruption of the winter 1732 1733 theatre season at the Theatre Royal, Drury Lane.
Until the disruption caused by the outbreak of the Wars of Independence in the early fourteenth century, most naval trade was probably coastal and most foreign trade was with England.
Shell vials were incubated 3 4 weeks or until cell disruption occurred.
But those who wait until the disruptions are unavoidable -- easy to do when governments do not mount a timely response -- will miss out on the huge advantages that technology offers.
Union officials hope to build on the momentum of the strike today and continue the labor disruption until the government backs down.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com