The cells were cultured at 37°C in a 5% CO2 in air atmosphere until confluent, and subcultured using a disaggregation assay with trypsin 0.1% and EDTA 0.01% in PBS (pH 7.5).
Unlike the culturing and passaging of most lines where the majority of cells survive disaggregation and go on to proliferate until the culture becomes confluent, during passaging in the NSA, the majority (>90%) of the cells die or do not further proliferate.
The standing biomass is increasing with an increasing age until the age of the climax phase and will decline in the following disaggregation phase.
Disaggregation in the Medicon was repeated four to six more times to completely disaggregate the tissue.
This digital disaggregation, or fragmentation, of a newspaper has, of course, severe economic implications.
In a world of increasing aggregation, it is disaggregation that often threatens meaning.
The word he came up with was "disaggregation".
Asked to characterize Washington's current approach to Europe, a senior State Department official recently responded with one word: "disaggregation".
In recent decades, those on the American left have tended to be preoccupied with disaggregation and difference.
In this era of political disaggregation, Labour is fragmenting more than most – to Ukip, the SNP, Plaid Cymru, and the Greens.
