Exact(5)
Our objective is to estimate the time after release until dilution with air of a combustible vapor results in the local concentration being everywhere below the fuel-lean flammability limit (or until the dilution of a toxic vapor results in the local concentration being everywhere tolerable for human occupation).
The NaCl content was gradually reduced by 1.25 g/L every 8 h until dilution resulted in removal of all NaCl.
RAPA stock solution was prepared by dissolving rapamycin in DMSO (25 mg/ml) and stored until dilution with PBS for intraperitoneal injection.
Peptide acetate salt powder was dissolved in phosphate-buffered saline (PBS) to 10 mM and aliquots were stored frozen until dilution in PBS, filtration, and use.
For dcausal(t*) to be correctly interpretable as an effect of receiving screening, we assume that after perhaps some initial fluctuations p1 t) -p0 t) is generally increasing or constant over time until dilution reduces z(t).
Similar(55)
This step was repeated until serial dilution is performed up to tube 2. A solution of 2.5 μl was extracted from tube 2 after the serial dilution to make the volumes constant.
All peptides were dissolved in distilled water and stored at −20°C or −80°C until further dilution in saline immediately prior to use.
Single cells were isolated by suspending a bulk of cells in PBS until a dilution of about 1 cell/0.5 µl was obtained.
As shown in Figure 5B, ΔFn 2706G decreased with decreasing product concentrations, whereas the ΔFn 7028C values slightly increased until a dilution factor of eight.
Dilutions of control samples identical to those in Figure 1A and B (containing PrPTSE and vehicle, no lichen extract) were detectable by immunoblotting until the dilution was increased beyond a factor of 100.
When used as antigen in a direct ELISA assay, purified proteins E and N were detected by the sera of two rabbits immunized with P. acanthamoebae until a dilution value of 1/256 while only background reaction is observed with pre-immune sera at this and lower dilutions (Fig. 3B,D).
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