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Cell lysates were stored at −80°C until chemical analysis.
Serum was stored at −20°C until chemical analysis.
The samples were centrifuged and stored at -70 °C until chemical analysis.
The powder was filtered through a 20-mesh sieve and stored in a drier until chemical analysis.
We collected urine into polypropylene specimen cups that had been lot tested for phthalate contaminants, refrigerated until processing, and stored at or below −20 °C until chemical analysis.
Cultures were run at D = 0.02 h−1 for at least five to six volume changes (one volume change is 50 h) and until chemical analysis of effluent showed constancy over at least two consecutive days.
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Sediment samples were kept frozen until the chemical analysis was undertaken.
Elutriates were stored at +4°C until the chemical analysis was undertaken.
The oil layers were separated and dried over anhydrous sodium sulfate, stored in hermetically sealed glass containers, and refrigerated at 4°C until the chemical analysis and cytotoxicity and leishmanicidal assays occurred.
The selected breast milk samples were thawed at room temperature for 12 hr, heated, and shaken for 30 min at 37°C to homogenize the samples, and then divided into smaller aliquots and refrozen at –20°C until further chemical analysis.
The serum was centrifuged, transferred to dark-colored acetone-washed glass bottles, frozen, and kept at –20°C until the start of the chemical analysis.
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