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The serum obtained was stored at −20°C until assessments.
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The right tibia was stored at − 20 °C until assessment of the biomechanical properties of cartilage was performed.
Until assessment of the accessibility of e-government Web sites accounts for all of the relevant laws, the understanding of levels of accessibility and compliance will be incomplete.
Cells were washed, resuspended in 1% paraformaldehyde in PBS, and stored in the dark at 4°C until assessment by FACS within 24 hours.
Mice were euthanized at P12, brains were harvested and shock frozen in ice cold (−20°C) 2-methylbutane and stored at −80°C until assessment of ventricular size or MBP immunocytochemistry (see below).
Processed samples were frozen at -80°C until assessment.
Serum samples were aliquoted and stored at -80°C until assessment.
Notes: 1) LOS = length of stay until assessment.
Samples were immediately centrifuged, and supernatants were stored at −80°C until assessment.
The tissue was taken at the end of protocol and stored at -80°C until assessment of caspase-3 activity.
The information on these covariates was updated from the start of the cohorts until assessment of the questionnaire addressing RLS.
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