Exact(7)
The egg water and chemicals were changed once a day until assays.
Blood samples were then processed to isolate serum and stored at −20°C until assays for testosterone were performed.
The medium fractions were collected every 5 or 10 min and frozen at −75°C until assays.
The separated plasma samples were stored in multiple aliquots in a freezer at −80°C until assays.
Bothrops jararaca, Lachesis muta lyophilized venoms, and anti-Lachesis or anti-Bothropic antivenom were provided from Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil, and stored at −20°C until assays.
The biopsy tissues were incubated immediately in Hanks' medium at 37°C, 5% CO2 for 30 min before storing at −80°C until assays for mast cell mediators were performed by the Immunopharmacology Group at the University of Southampton.
Similar(53)
Samples were kept on ice until assayed.
Plasma and lymphocytes were kept frozen at −80°C until assayed.
until assayed for cytokines.
The tubes containing samples were stored at −70°C until assayed.
Urine samples were stored at −20°C until assayed.
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