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TG were immediately frozen on dry ice and stored at −80 °C until assay.
Plasma was kindly provided by EL-Minia Hospitandof Psychiatric medicine and kept frozen until assay.
Serum was aliquoted into multiple Eppendorf tubes and frozen at ≤−20 °C until assay.
Samples were collected, separated, and stored at –70°C until assay.
Samples were stored at −80°C until assay.
Tissue samples were frozen in −80°C until assay.
Plasma for these latter three assays was separated immediately, then frozen at −80°C until assay.
Plasma was immediately isolated and stored at −80°C until assay.
Peripheral blood mononuclear cells (PBMC) were separated and cryopreserved in liquid nitrogen until assay time.
Tissue samples were immediately frozen on dry ice and were stored at −80°C until assay.
The supernatant was stored in aliquots at −80°C until assay.
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